AJLHTS JOURNAL – VOL 2 No 4

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doi.org/10.59708/ajlhts.v2i4.2326

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Performance Of Enzyme Linked Immunosorbent Assay and Rapid Screening Techniques for detection Of Transfusion Transmitted Infections among blood donors

Esan Ayodele Jacob, Owoseni M.F, Ibijola A, Omisakin C.T, Omoniyi D.P, Oyedele Titilayo and Fasakin Kolawole

^1,2,3,4,5,7 Department of Haematology and Blood Transfusion Science, Federal Teaching Hospital, Ido-Ekiti, Nigeria.

⁶Department of Medical Laboratory Science, Achiever’s University, Ondo State, Nigeria.

*Corresponding Author: ESAN Ayodele Jacob, Department of Haematology and Blood Transfusion Science, Federal Teaching Hospital, Ido-Ekiti, Nigeria. Orcid number: 0000-0002-3350-5991, e-mail: ayodelejacob4u@gmail.com, ayodelejacob4u@fethi.gov.ng,+2348035477756

Abstract

Introduction: Blood transfusion is associated with many risks, especially exposure to a transfusion-transmitted infections (TTIs). Lack of effective diagnostic techniques that can identifying the infection at window period contributes to the major health problem in most developing countries. The aim of this study was to compare diagnostic performance of enzyme linked immunosorbent assay (ELISA) and rapid screening techniques for detection of transfusion-transmitted infections (TTIs) among blood donors

Methodology: This study was conducted in the Department of Haematology and Blood Transfusion Services Federal Teaching Hospital, Ido-Ekiti, Nigeria from February 2021 to July 2023. A total of 3150 eligible blood donors that were screened fit for blood donation using rapid kits were subjected to ELISA screening technique. Questionnaire was used to inquire about risk factors for transfusion transmissible infection among blood donors and to sort the consent of all the blood donors before collecting their blood sample for study

Result: A total of 3150 blood donors that were screened negative for TTIs (HbsAg, HCV, HIV and syphilis) by rapid test kits were recruited for the study. Out of 3150 blood donors that were re-screened for TTIs by ELISA techniques, 34 (1.08%), 50 (1.59%), 07 (0.22%) and 13 (0.41%) were sero-positive for HbsAg, HCV, HIV and syphilis respectively. In general, the percentage seroprevalence of TTIs in our study was 104/3150 (3.3%), HCV had the highest prevalence of TTIs and Blood group O Rh D positive has the highest frequency among the study population

Conclusion: This study concluded that rapid test did not show any promising results when compared to ELISA. This study suggested that rapid test kits should not be recommended in transfusion center solely for screening blood donors for TTIs. There is need for combination of rapid test kits along with ELISA technique in the diagnosis of TTIs for blood donors. We advocate for legislation to make screening with ELISA mandatory for TTIs in all blood transfusion center and other health facilities in the country

Key words: Blood transfusion, Transfusion transmissible infection, Blood donors, Rapid kits, ELISA technique

doi.org/10.59708/ajlhts.v2i4.2331

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Red cell parameters, iron, vitamin B₁₂ and folate levels of pulmonary tuberculosis patients attending clinic at General Hospital, Akamkpa, Cross River State, Nigeria

Akpan, P. A., Ogar, R. O., Nyong, E. A.

1. Department of Haematology and Blood Transfusion Science,
   Faculty of Medical Laboratory Science,
   University of Calabar, Nigeria.
2. Department of Medical Laboratory Science (Haematology Unit), College of

Health Technology, Calabar.

* Corresponding author: apu0520@unical.edu.ng; +2348027321305

Abstract

Introduction: Tuberculosis caused by Mycobacterium tuberculosis, is a disease of public health importance, characterized by a chronic granulomatous inflammation in humans. In this study, red cell and iron parameters as well as vitamin B₁₂ and folate levels were assessed with a view to investigate the presence or absence of iron deficiency and/or vitamin B₁₂ and folate deficiency in tuberculosis disease

Methods: Fifty (50) male and female tuberculosis patients within the ages of 15-60 years and attending clinic at the General Hospital, Akamkpa were enrolled in this study. Fifty apparently healthy and mantoux-negative subjects who were age and gender matched and resident in Akamkpa served as control. Ethical approval and informed consent were obtained from the Cross-River State Ministry of Health and all participants. Demographic information was obtained by face-to-face interview. Diagnosis of TB was by the Ziehl-Neelsen technique. Red cell and iron parameters, vitamin B12 and folate levels were determined by standard methods. Data were analyzed using student t-test on statistical package for social sciences version 21and a P value less than or equal to 0.05 is considered significant.

Results: The mean age of TB patients (34.7±10.4 years) is comparable to that of the control (32.4±8.6 years) with more males (56%) affected than females (44%). Fifteen (30%) of the TB patients have primary level of education with 31 (62%) and 4 (8%) having attained secondary and tertiary levels. The level of education for the control was 12 (24%), 22 (44%) and 16 (32%) for primary, secondary and tertiary respectively. The TB patients were farmers 20 (40%), traders 15 (30%), students 9 (18%) and civil servants 6 (12%) while the controls consisted of 14 (28%) farmers, 16 (32%) traders, 12 (24%) students and 8 (16%) civil servants. The packed cell volume and haemoglobin concentration of TB patients (0.34±0.05 L/L and 121.4±11.3 g/L) was significantly lower (p < 0.05) than 0.41±0.04 L/L and 145.2±13.1 g/L for the control. The red cell count and mean cell volume of TB patients (4.55±0.73 x 10 ¹²/L and 79.21±2.40 fl) was comparable (p > 0.05) to control values (4.84±0.54 x 10 ¹²/L and 80.18±1.30 fl). Mean cell haemoglobin and mean cell haemoglobin concentration of TB patients was 27.58±2.12 pg and 34.37±1.43 g/dl, which was significantly lower than 29.72±2.24 pg and 35.73±1.38 g/dl obtained for control. The serum iron of TB patients (47.90±6.25µg/dl) was significantly lower (p = 0.001) than control value 109.03±8.56µg/dl. The total iron binding capacity (188.05±33.01µg/dl) and transferrin saturation with iron (28.00±4.54 %) were significantly lower (p = 0.001) for TB patients versus control (252.28±36.30µg/dl and 42.46±6.23%). Serum ferritin of TB patients (345.30±82.61 ng/ml) was significantly higher (p = 0.001) when compared to the value for control (108.62±28.50 ng/ml). Vitamin B₁₂ and folate levels (245.3739.62 ng/L and 353.3457.06 µg/l) were significantly lower (p = 0.001) for TB patients when compared with control (550.2082.33 ng/L and 681.9397.36µg/l) though within the reference values.

Conclusions: This study has shown a lower packed cell volume and haemoglobin concentration in tuberculosis disease indicating the presence of anaemia. An alteration in iron metabolism and increased iron stores has also been demonstrated ruling out iron deficiency anaemia. Vitamin B12 and folate levels though lower for TB patients, are within the reference range thus excluding megaloblastic anaemia. Tuberculosis shows a normocytic normochromic anaemia which is typical of anaemia of chronic disease and inflammation.

Keywords: Tuberculosis, iron, vitamin B_12, folate, Akamkpa

doi.org/10.59708/ajlhts.v2i4.2333

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Utility of World Health Organization Haematological Toxicity Scale in the Assessment of Smokers in Port Harcourt, Nigeria.

 Akabs Ibise Jewel, Theresa Awortu Jeremiah, Zaccheaus Awortu Jeremiah, 

¹Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Rivers State University, Port Harcourt,Nigeria.

²Department of Microbiology, Parasitology and Immunology, Faculty of Medical Laboratory Science, Federal University Otuoke, Otuoke, Nigeria.

Corresponding Author:

Prof Z.A.Jeremiah.

e-mail: zaccheaus.jeremiah@ust.edu.ng

Phone: +2348034045636

Running Title: WHO haematological toxicity scale and evaluation of smokers

Abstract

Introduction: This study was aimed at evaluating some haematological parameters among smokers in Port Harcourt, Rivers State and to ascertain if the effect of smoking can be assessed using the World Health Organization Haematological toxicity scale.

Materials and Methods: A cross-sectional study which involved 100 individuals (50 smokers and 50 non-smokers) within the ages of 20-45 years was used. Blood samples were collected by venipuncture into an EDTA anticoagulant bottle for haematological analysis of selected haematology parameters. The samples were processed using haematology auto analyser- SYSMEX KX-21. Statistical analysis was done using Graph Pad prism version 8.02 for windows. Comparisons of mean and standard deviation were made for the various parameters using student’s t-test.

Results: The mean values of haemaglobin for smokers (14.50 ± 1.773 g/dL) was significantly elevated when compared with 11.74 ±1.15 g/dL in non-smokers. (p≤ 0.0001). There was no statistically significant difference in the mean values of leucocyte count in smokers compared to non-smokers (p ≥ 0.05). The mean value of granulocyte count in smokers (4.52 ± 1.28 x 10⁹/L) was significantly higher than that of non-smokers (3.81 ±.0.72 x10⁹/L) (p ≤ 0.0008). The mean platelet count of smokers (236.0 ± 64.65×10⁹/L) was significantly raised when compared with that of non-smokers (217.7 ± 42.71×10⁹/L). In the control group, 8% were found to be anaemic corresponding to Grade 2 of the WHO toxicity scale (8-9.4g/dL) whereas all of the smokers were one hundred percent (100%) in grade 0 (≥ 11.0 g/dL). For the white blood cell count, majority of the control subjects were 90% grade 2 (3.0-3.9×10⁹/L) whereas among the smokers, they were 100% in grade 0. For the platelets, all the control subjects were 100% grade 0 (≥×10⁹/L) while 2% of the smokers (test) fell into grade 1 (75-99×10⁹/L) of the WHO toxicity scale ( ie mild thrombocytopenia).

Conclusion: We concluded that smoking causes a significant elevation of haemogobin and granulocytes and at the same time elevated platelet counts whereas 2% of smokers fell into grade 1 of the toxicity scale. This effect may be more pronounced if a longer duration of smoking and a larger sample is considered in further studies.

Keywords: WHO toxicity scale; Haematological Parameters,smokers,

doi.org/10.59708/ajlhts.v2i4.2337

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Duffy Red Cell Antigen Phenotype among Indigenous Pregnant Women attending Antenatal Clinic at Federal Teaching Hospital Gombe,   Nigeria.

Ahmed M. Gaji, Ann Ogbenna, Ahmed I. Girei, Kasim M. Pindiga, Sani Adamu, Saleh Yuguda, Ademola Adewoyin , Davies N.O.

¹Department of Haematology and Blood Transfusion, College of Medicine, University of Lagos,Nigeria

²Department of Haematology and Blood Transfusion Federal Teaching Hospital Gombe, Gombe State, Nigeria.

³Department of Chemical Pathology, Federal Teaching Hospital Gombe, Gombe State, Nigeria.

Curresponding Author: mohammedgaji2@gmail.com

Abstract

Background and Objectives: Duffy (FY) blood group system is implicated in transfusion incompatibilities and haemolytic disease of the foetus and newborn. The primary objective was to determine the Duffy phenotype among the indigenous pregnant women in Gombe, Gombe State, Nigeria. 

Materials and Methods: This was a cross sectional study where simple random sampling was employed on consented participants. Two hundred and fifty nine pregnant women attending antenatal clinic at Federal Teaching Hospital Gombe were randomly recruited into the study. Three milliliters (3mls) of blood was taken, and Duffy antigens typed by standard tube technique (LORNE LABORATORY UK).

Results: Among the Indigenous tribe, the percentage of Fy(a+b+) was seen in 2.2% of Fulani and 3.4% of Tangale, Fy(a+b-) phenotype was seen in 4.3% of Tangale, 6.8% of Fulani,9.5%  of Tera, 10.3% of Hausa and 10.5% of Waja.  Fy(a-b+) phenotype was seen in 5.3% of Waja, 7.6% of Fulani,8.7% of Tangale, 9.5% of Tera and 12.5% of Bolawa. Fy(a-b-) phenotype was seen in 2.4% of Tula,6.4% of Bolawa,7.3% of Waja, 7.8% of Tera, 17.8% of Tangale, 11.8% of Hausa and 46.5% of Fulani. 84.6% of the study population had the null Duffy phenotype.

Conclusion:  The research showed the phenotypic distribution of Duffy blood group among the study participants with relatively high percentage of null Duffy phenotype hence possible risk of alloimmunisation.

Key words: Duffy phenotype, Haemolytic disease of foetus and newborn, plasmodiasis.

doi.org/10.59708/ajlhts.v2i4.2338

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Performance evaluation of the Hemotype SC^TM   rapid test for the diagnosis of sickle cell disease

Diallo Issiaga, Adje Missa Louis, Salifou Talassone Bangoura, Yayo Aye Mireille  Kadio Jean-Jacques Olivier Kadio, Sidibé Sidikiba, Sawadogo Duni

¹ Faculty of Health Sciences and Techniques, Gamal Abdel Nasser University, Conakry, Guinea

² Hematology Laboratory University Hospital Yopougon Abidjan

³UFR Pharmaceutical and Biological Sciences Université UFHB Abidjan

⁴ Center for Research and Training in Infectious Diseases of Guinea (CERFIG)

Email address:

issiagahady@gmail.com (Issiaga Diallo*), adjemissa@gmail.com (Adje Missa Louis), talassone.bangoura@cerfig.org (Salifou Talassone Bangoura), yayoaye@yahoo.fr (Yayo Aye Mireille), olivier.kadio@cerfig.org (Kadio Jean-Jacques Olivier Kadio), layesidikiba@gamail.com (Sidibé Sidikiba) dunisawadogo@gmail.com (Sawadogo Duni)

Abstract

Introduction: The use of Rapid Orientation Diagnostic Tests (“RODTs”) could play an important role in the mass screening of certain diseases. It saves a great deal of time while managing the flow of samples and the difficulties associated with their storage. This study aimed to identify the impact of the delay between sampling and analysis on the analytical performance of the Hemotype RODT SC^TM.

Methods: This was a descriptive cross-sectional study conducted over one month. The Immunology-Hematology Department of the CHU de Cocody served as the study setting. The study population consisted of sickle cell patients and hemoglobin C carriers of all ages and sexes. We performed hemoglobin electrophoresis on all samples on the day of collection (D0) and the Hemotype SC^TM rapid test on D0, D2, D4, and D6. Sensitivity and specificity were used to determine analytical performance, and the chi2 test was used to compare qualitative variables.

Results: The study involved 102 patients with a median age of 16 years (IQR: 9-24). Half of the participants (56.96%) were SSFA2 homozygous sickle cell disease major. On average, 87.1% of RODT results were valid from D0 to D6. At D0, the RODT had a sensitivity of 75% and a specificity of 81.40% for Hb A; Hb S had a sensitivity of 79.80% and a specificity of 100%; Hb C had a sensitivity of 35.71% and a specificity of 98.65%. The sensitivity of Hb C and the specificity of Hb A had increased statistically significantly over time. Cross-referencing the RODT results from D0 to D6 with those of electrophoresis showed a concordance of 86.95% for the AA phenotype, 79.52% for AS; 88.52% for SS; and 95.45% for SC.

Conclusion: The HemoTypeSC™ test, with a sensitivity of 75% and specificity of 98% is suitable in certain areas where access to electrophoresis is limited.  This performance is not impaired on samples stored for 6 days.

Keywords: Evaluation, HemoTypeSC, Sickle cell disease, Hemoglobin