doi-org-10-59708-ajlhts-v3i2-2419
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Abstract
Background: Glucose-6-phosphate dehydrogenase (G6PD) is crucial
in protecting red blood cells from oxidative stress, while Krüppellike factor-1 (KLF-1) regulates erythropoiesis and haemoglobin.
Understanding how the bi-herbal formulation modulates these
genes could provide valuable insights into its potential as a
therapeutic intervention for anaemia.
Aim: The aim of this study was to determine the effect of the biherbal formula of Picralima nitida and Cymbopogon citratus aqueous
leaf extracts on glucose-6-phosphate dehydrogenase (G6PD) and
Krüppel like factor-1 (KLF-1) gene expressions in phenyl-hydrazine
induced anaemia in albino Wistar rats.
Methods: A total of thirty (30) adult male albino Wistar rats were
divided into six (6) groups; A, B, C, D, E and F representing
control, a phenyl hydrazine group, ferrous sulphate group, phenyl
hydrazine + 100mg/kg bi-herbal formulation of Picralima nitida
and Cymbopogon citratus, phenyl hydrazine + 200mg/kg bi-herbal
formulation of Picralima nitida and Cymbopogon citratus and phenyl
hydrazine + 400mg/kg bi-herbal formulation of Picralima nitida
and Cymbopogon citratus respectively. mRNA of G6PD and KLF-1
were determined using a polymerase chain reaction. Data obtained
was analysed by the Statistical Package for Social Science (SPSS)
Software.