Effect of Storage Temperature on D-Dimer Stability in Different ABO Blood Groups

doi-org-10-59708-ajlhts-v3i2-2427

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Abstract
Background: D-dimer is the end product of fibrin degradation formed
during the disintegration of blood clots and fibrin networks. It is an
invaluable analyte in evaluating thrombin and plasmin activity and
is specific for fibrin derivatives. This study evaluated the effects of
storage temperatures on D-dimer stability in different ABO blood
types stored over a defined period of time.
Materials and Methods: The study was performed with 40 samples
collected randomly from apparently healthy male and female
undergraduate students of Rivers State University, Port-Harcourt,
aged between 17 and 45 years. Two milliliters (2ml) of whole blood
were collected from each participant with the specified ABO blood
type into ethylene diamine tetra acetic acid (EDTA) anticoagulant
bottle, and plasma was separated and stored at two different
temperatures of +6o
Cand +25o
C for a period of 24 hours and 48 hours
respectively. Following the manufacturer’s instructions, the plasma
blood sample was analyzed using the Standard F-D-dimer FIA
reagent and SD-Biosensor analyzer. Data obtained were analyzed
statistically using the Statistical Package for Social Science (SPSS)
version 23, and results were considered significant at p< 0.05.

Hepatitis E Virus Infection among Antenatal Attendees in Ekiti State, Nigeria: Awareness and Prevalence

doi.org/10.59708/ajlhts.v3i2.2423

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Abstract
Background: Hepatitis E virus (HEV) infection is an enteric viral
infection and a global problem of increasing threats among pregnant
women with an estimated annual burden of 20 million cases, 70,000
deaths, and 3,000 stillbirths. This study aims to determine the level
of awareness of HEV infection, its prevalence, pregnancy risks, and
transmission mode among antenatal attendees in Ekiti State.
Methods: This is a dual-center study performed at the antenatal
clinics of the study sites over a period of fifteen months following
ethical approval. Socio-demographic, pregnancy-related, and HEV
awareness data of 300 antenatal attendees were collected during
counseling sessions at the clinics. A Biopanda rapid test device
was used to test for HEV antibodies. Data were analyzed using
the descriptive statistics of the SPSS version 21 software, and the
association between hospital and HEV awareness and HEV mode
of transmission variables was tested with Chi-square analysis.

Association of Lewis blood group with Helicobacter pylori Infection amongst Undergraduates of Rivers State University, Port Harcourt, Nigeria.

doi-org-10-59708-ajlhts-v3i2-2421

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Abstract
Background: There are possibilities that the presence of a particular
blood group antigen can predispose an individual to certain diseases.
It is therefore necessary to carry out studies in relation to this, and
the presence of Lewis blood group antigen has been associated with
susceptibility to Helicobacter pylori infection. The aim of the study is
to determine the association of Lewis blood group with Helicobacter
pylori infection amongst undergraduates of Rivers State University,
Port Harcourt, Nigeria.
Method: This cross-sectional study was aimed to elucidate the
association between Lewis blood group and Helicobacter pylori
among one hundred (100) male and female undergraduates of
Rivers State University recruited randomly from different ethnic
groups in Nigeria. Conventional tube method was used for Lewis
blood grouping, and serology for the Helicobacter pylori infection
using Top Tech Rapid Diagnostic Test Kit. Statistical analysis was
by percentage calculation and the use of Graph Pad Prism version
8 Statistical Package to determine Odd Ratios, Relative Risk and
Likelihood Ratio; p-value of <0.05 was considered statistically
significant.

Effect of a Bi-Herbal Formula of Picralima Nitida and Cymbopogon Citratus aqueous leaf extracts on Glucose-6-Phosphate Dehydrogenase and Krüppel-Like Factor- 1 Genes In PhenylHydrazine induced Anaemia in Albino Wistar Rats

doi-org-10-59708-ajlhts-v3i2-2419

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Abstract
Background: Glucose-6-phosphate dehydrogenase (G6PD) is crucial
in protecting red blood cells from oxidative stress, while Krüppellike factor-1 (KLF-1) regulates erythropoiesis and haemoglobin.
Understanding how the bi-herbal formulation modulates these
genes could provide valuable insights into its potential as a
therapeutic intervention for anaemia.
Aim: The aim of this study was to determine the effect of the biherbal formula of Picralima nitida and Cymbopogon citratus aqueous
leaf extracts on glucose-6-phosphate dehydrogenase (G6PD) and
Krüppel like factor-1 (KLF-1) gene expressions in phenyl-hydrazine
induced anaemia in albino Wistar rats.
Methods: A total of thirty (30) adult male albino Wistar rats were
divided into six (6) groups; A, B, C, D, E and F representing
control, a phenyl hydrazine group, ferrous sulphate group, phenyl
hydrazine + 100mg/kg bi-herbal formulation of Picralima nitida
and Cymbopogon citratus, phenyl hydrazine + 200mg/kg bi-herbal
formulation of Picralima nitida and Cymbopogon citratus and phenyl
hydrazine + 400mg/kg bi-herbal formulation of Picralima nitida
and Cymbopogon citratus respectively. mRNA of G6PD and KLF-1
were determined using a polymerase chain reaction. Data obtained
was analysed by the Statistical Package for Social Science (SPSS)
Software.

Expression of Serum Secretory Phospholipase A2 and Its Association with Clinical Characteristics in Sickle Cell Anaemia Patient: A Case-Control Study in Ghana

doi-org-10-59708-ajlhts-v3i2-2418

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Abstract
Background: Secretory phospholipase A2 (sPLA2) has been
implicated in the pathogenesis and complications of SCA, however
its pathophysiology is not fully understood. This study determined
the levels of sPLA2 in SCA patients and its association with different
clinical characteristics.
Materials and Methods: This case-control study recruited a total of
90 participants, 45 SCA patients and 45 control subjects. Venous
blood sample and serum were used to estimate full blood count
(FBC) and sPLA2 respectively. The collected data was organised in
Microsoft excel and analysis done with Statistical Package for the
Social Sciences (SPSS-version 26). Statistical significances was set at
P<0.05.
Results: The mean values of haematological indices showed
significant differences between the control and sickle cell anaemia
groups: RBC [p<0.001], Hb [p<0.001], HCT [p<0.001], WBC [p<0.001],
MCV [p<0.001], RDW-SD [p<0.005], and PLT [p<0.001]. The mean
sPLA2 concentration was significantly higher in SCA cases than
in the control group [(60.67±19.43)µg/L vs. (23.18±4.32) µg/L;
p < 0.001]. The sPLA2 concentrations between SCA patients with
respect to the use of Hydroxyurea treatment showed no significant
differences, [p=0.581].
Conclusion: Secretory phospholipase A2 was elevated in SCA
patients. Those who presented with vaso-occlusive recorded the
highest concentrations of sPLA2. White blood cell count, mean cell
haemoglobin concentration, Red Cell Distribution width-standard
deviation and platelets were significantly elevated in SCA whereas
RBC, Hb, and HCT were low. RDW-SD showed significant variations
in the different clinical presentations of SCA. RDW-SD and sPLA2
levels revealed a negative correlation in SCA.

Modulation of thrombocytes, thrombopoietin, thrombopoietin receptors and ribonucleic acid expression with Jatropha tanjorensis using albino Wistar rats

doi-org-10-59708-ajlhts-v3i2-2417

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Abstract
Background: Jatropha tanjorensis leaf extract possesses some
bioactive compounds with potential medicinal properties. Its role
in thrombopoiesis and the associated molecular pathways could
offer useful insights into its therapeutic potential in conditions
related to platelet disorders. This study is aimed at investigating
the modulatory effects of this leave extract on platelet functions,
thrombopoietin (TPO), cyclic myeloproliferative leukaemia virus
oncogene(c-MPL) and messenger ribonucleic acid (RNA) in the
modulation of thrombopoiesis using male albino Wistar rats.
Materials and Methods: A total of twenty (20) male adult albino
Wistar rats weighing between 150g-250g were selected into four (4)
groups of five rats each. The groups were control groups(A), treated
group B(1000mg/kg leave extract, treated group C(2000mg/kg
leave extract, and treated group D(4000mg/kg leave extract. Platelet
counts and platelet indices were done using a three-part Sysmes
Haematology autoanalyzer. RNA extraction and semi-qualitative
polymerase chain reaction (PCR) were used to isolate from rat bone
marrow; complementary DNA was synthesized and subsequently
amplified. Gel electrophoresis was used to determine the bands of
the genes.

Relationship between platelet count and ferritin in blood donors in Lagos, Nigeria

doi-org-10-59708-ajlhts-v3i2-2416

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Abstract
Background: Iron deficiency anaemia is a major reason donors are deferred from donation. Iron deficiency is an important donor health concern
with adverse consequences even without anaemia. However, iron-deficient
donors are usually undetected using the current screening of haemoglobin
methods in most Nigerian blood centres. Thrombocytosis has been associated with iron deficiency, and thrombocytopenia has also been reported in
severe anaemia. This study assessed the relationship between platelet and
ferritin in blood donors.
Materials and Methods: This was a cross-sectional study of blood donors
attending the Lagos University Teaching Hospital (LUTH) between March
1st, 2019, and July 31st, 2019. A proforma was used to obtain the sociodemographic information. A donation sample was collected for a complete
blood count and ferritin assay. Data was analysed using SPSS version 17.0.
Results: Data from 313 blood donors with an age range between 18 years
to 58 years and a mean age of 32.1 ± 9.3 years were analysed. The mean
platelet count was 207.6 ± 73.8 X 109
/L while the median (Inter Quartile
Range) for ferritin was 102.7 ng/mL(62.4 ng/Ml -182.0 ng/mL) respectively. There was no statistically significant relationship between platelet count
and ferritin among all categories of blood donors (where is the correlation
coefficient?). Regular blood donors had significantly lower serum ferritin
levels than first-time donors (p = 0.0356). There was, however, no statistically significant difference between regular donors and first-time donors
with respect to platelet levels (P= 0.1107)

Co-occurrence of atypical chronic myeloid leukemia and prostate cancer in a 75-years old male

doi-org-10-59708-ajlhts-v3i2-2415

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Abstract
Atypical chronic myeloid leukemia (aCML) is a rare form of myelodysplastic syndrome/myeloproliferative neoplasm. This report
presents a case of aCML in a 75-year-old man, diagnosed based on
cytologic evidence of neutrophilia, presence of neutrophil precursors, thrombocytopenia on complete blood count and microscopy,
and granular hyperplasia and dysgranulopoiesis on myelogram.
The patient was in good clinical condition with isolated splenomegaly. With hydroxyurea, the hyperleukocytosis was controlled, and
the splenomegaly disappeared. The lymphadenopathy identified
on a CT scan led to the diagnosis of prostate cancer, while the general health condition of the patient was impairing.
Key words: atypical chronic myeloid leukemia; prostate cancer;
hydroxyurea.

Effect of aqueous leaf extract of Moringa oleifera on some complete blood count and some oxidative stress markers of stress-induced Albino Wistar rats.

doi-org-10-59708-ajlhts-v3i2-2408

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Abstract
Introduction: Moringa oleifera is a nutritive plant that is used to
treat malnutrition in underdeveloped nations due to its nutritional
benefits. It contributes significantly to humans’ diet and is a good
source of protein, carbohydrates, vitamins, and minerals. The leaves
of Moringa oleifera contain phytochemicals that may impact one’s
health. This study aimed to determine the effect of administering
feed formulated from Moringa oleifera leaves on some hematological
Parameters and oxidative stress markers of Gasoline vapour stressinduced Albino Wistar rats.
Materials and methods: Fifty-eight (58) age-matched, apparently
healthy male Albino Wistar rats were obtained from the animal
holding unit of the Anatomy Department, University of Benin,
Benin City, Nigeria. The rats were housed in stainless steel cages
measuring 4 ft x 2 ft x 2 ft, partitioned into four compartments at
the same facility. Automated ERMA haematology autoanalyzer
PCE-210 was used to analyze the haematological parameters
while Enzyme- linked immunosorbent assay (ELISA) was used to
analyze Malondialdehyde (MDA), Catalase (CAT) and Superoxide
dismutase (SOD) for oxidative stress.