doi-org-10-59708-ajlhts-v3i2-2417
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Abstract
Background: Jatropha tanjorensis leaf extract possesses some
bioactive compounds with potential medicinal properties. Its role
in thrombopoiesis and the associated molecular pathways could
offer useful insights into its therapeutic potential in conditions
related to platelet disorders. This study is aimed at investigating
the modulatory effects of this leave extract on platelet functions,
thrombopoietin (TPO), cyclic myeloproliferative leukaemia virus
oncogene(c-MPL) and messenger ribonucleic acid (RNA) in the
modulation of thrombopoiesis using male albino Wistar rats.
Materials and Methods: A total of twenty (20) male adult albino
Wistar rats weighing between 150g-250g were selected into four (4)
groups of five rats each. The groups were control groups(A), treated
group B(1000mg/kg leave extract, treated group C(2000mg/kg
leave extract, and treated group D(4000mg/kg leave extract. Platelet
counts and platelet indices were done using a three-part Sysmes
Haematology autoanalyzer. RNA extraction and semi-qualitative
polymerase chain reaction (PCR) were used to isolate from rat bone
marrow; complementary DNA was synthesized and subsequently
amplified. Gel electrophoresis was used to determine the bands of
the genes.