doi-org-10-59708-ajlhts-v3i2-2427
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Abstract
Background: D-dimer is the end product of fibrin degradation formed
during the disintegration of blood clots and fibrin networks. It is an
invaluable analyte in evaluating thrombin and plasmin activity and
is specific for fibrin derivatives. This study evaluated the effects of
storage temperatures on D-dimer stability in different ABO blood
types stored over a defined period of time.
Materials and Methods: The study was performed with 40 samples
collected randomly from apparently healthy male and female
undergraduate students of Rivers State University, Port-Harcourt,
aged between 17 and 45 years. Two milliliters (2ml) of whole blood
were collected from each participant with the specified ABO blood
type into ethylene diamine tetra acetic acid (EDTA) anticoagulant
bottle, and plasma was separated and stored at two different
temperatures of +6o
Cand +25o
C for a period of 24 hours and 48 hours
respectively. Following the manufacturer’s instructions, the plasma
blood sample was analyzed using the Standard F-D-dimer FIA
reagent and SD-Biosensor analyzer. Data obtained were analyzed
statistically using the Statistical Package for Social Science (SPSS)
version 23, and results were considered significant at p< 0.05.