Abdulwaheed Adewale Ademosun, Musa Abidemi Muhibi, Tesleemah Oluwakemi Davies-Folorunso, Olufemi David Olaniyi, Nuryn Abdulganiy, Fatimat Adewumi Adedapo-Ismail, Yekeen Adebisi Kosamat, And Mathew Folaranmi Olaniyan
AJLHTS: Original Paper DOI – https://www.ajlhtsonline.org/doi-org-10-59708-ajlhts-v2i3-2317/
Abstract
Introduction: Platelets are fragments of megakaryocytes circulating
in the blood and its concentrates are therapeutic in substantial
bleeding disorders. Efforts to ensure adequate product quality
are required due to their short lifespan and lack of robustness. A
descriptive longitudinal laboratory-based study was adopted in this
study. The study aimed at determining platelets survival in stored
platelet concentrates and evaluating thromboxane A2 for platelets
function.
Materials and Methods: Platelet concentrates were prepared
manually using buffy coat, where about 50ml of concentrates
suspended in plasma were allowed to rotate and agitate continuously
on platelet agitator at room temperature (20-240C). Aliquots of
4ml each was collected serially for 9 days (day 0 to day 8) from 10
different platelet concentrates collected from 7 male blood donors
and 3 female blood donors with age (mean± SD: 36 ± 7.14 years),
weight (mean± SD: 66.8 ± 6.01kg), height (mean± SD: 163 ± 4.57cm).
The samples were analyzed for platelets count, platelet distribution
width (PDW), mean platelet volume (MPV), platelet-large cell
ratio (P-LCR), using Automated Haematology analyzer (Sysmex
XP-300) and thromboxane A2 using standard ELISA technique.
Data analysis was carried out using mean, standard deviation as
descriptive statistics and logistic regression as inferential statistics;
and p <0.05 was considered as evidence of statistical significance.
Results: Socio-demographic characteristics had no effect on all
parameters estimated. There is variation in platelet count and
platelet indices values in stored platelet concentrates compared with
the baseline values and the survival of platelets in stored platelet
concentrates was relatively stable till day 4 after preparation but
depreciation surfaced from day 5 to day 8 compared to baseline
values. The study also showed that the degree of deterioration of
thromboxane A2 was highly significant at day 3 (p<0.05) while the best duration of storage for platelet concentrates in the study area
is the first 3 days, though storage up to day 5 is acceptable (p>0.05).
Conclusion: This study confirms thromboxane A2 as a marker
for platelet functionality. The best duration of storage for platelet
concentrates in the study area is the first two (2) days when no
significant deterioration was observed.
Keywords: Platelet count, Platelets survival, Platelet concentrates,
In-vitro assessment, Thromboxane A2.