INSTRUCTION FOR AUTHORS

Download PDF - 86 Downloads

 
 

Thank you for your interest in African Journal of Laboratory Haematology and Transfusion Science (AJLHTS). Authors should kindly note that submission implies that the content of the manuscript has not been published or submitted for publication elsewhere except as a brief abstract in the proceedings of a scientific meeting or symposium. 

Submitted manuscript for publication should be presented as follows

Your manuscript: this can be a single file including text, figures, and tables. All required sections should be contained in your manuscript, including abstract, introduction,

Download PDF - 86 Downloads

 
 

APPRECIATION

 

The AJLHTS Editorial Board sincerely appreciates all the manuscript reviewers for Volume 1(issue 1,2,3 and 4) 2022 

Prof Olutayo I. Ajayi University of Benin, Nigeria

Prof Josephine Akpotuzor – University of Calabar, Nigeria

Dr Nancy Ibeh – Nnamdi Azikiwe University, Awka, Nigeria

Dr Eze, Evelyn Mgbeoma – Rivers State University, Port Harcourt, Nigeria

Dr Ben Eledo – Federal Medical Centre, Bayelsa, Nigeria

Dr Alhaji Bukar – University of Maiduguri, Nigeria

Dr Marcus Chilaka – University of Bradford, United Kingdom

Prof. M. A. Muhibi – Edo State University, Uzairue, Nigeria

Prof. Z.A. Jeremiah – Rivers State University, Port Harcourt, Nigeria

Dr Rose Amaechi – Ambrose Ali University, Ekpoma, Nigeria

Dr Okezie Okamgba – Abia State University, Uturu, Nigeria

Dr Solomon Umukoro – Medical Research Council, The Gambia, West Africa

 

Challenges of bone marrow transplant for sickle cell disease in resource limited setting

Download PDF - 86 Downloads

 
 

Chisara Sylvestina Okolo and Ayodeji Olusola Dickson Olayanju

AJLHTS: Original Paper

 

Summary 

Sickle cell disorders (SCD) are a group of inherited disorders that results from both parent being carriers, haemoglobin AS resulting in haemoglobin (SS), causing anemia, infections, pain, stroke, priapism, sequenstration crisis, multiple organ dysfunctions etc. There are several ways of managing sickle cell disorder but the best way, which is considered a gold standard cure for the disease is a successful bone marrow transplant of haematopoietic stem cells (HSCT). Bone marrow (BM), Peripheral blood (PBSC) and Umbilical cord blood (UCB) are rich in stem cells. In order to have a good bone marrow transplant, without graft rejection, the laboratory plays a vital role especially in compatibility testing of donor and recipients at the various stages of the transplant, which includes initial stage, during the transplant and follow up testing to ensure tolerance to the new graft and testing for chimerism. There are various types of transplant which include Autologous, Allogeneic (Sibling/Unrelated Donor), Parent or relative, etc. The various pre transplant tests includes: Sickling test, High Performance Liquid Chromatograph (HPLC), Genetic studies, RBC Indices Hb 6-9 gm/dL, Peripheral Smear, Retic count Reticulocytosis, ESR, Bone marrow analysis. Post-transplant test Includes Engraftment Analysis, Complete Blood counts, kidney function, liver function, Cholesterol, HIV, Hepatitis B, Hepatitis C, additional infectious studies (Endemic testing), Urinalysis etc. Haemopoietic transplantation challenges in a resource limited settings like Nigeria are enormous and they includes: Danger of serious illness associated with donor-to-patient stem cell transplant, lack of appropriate donors, Graft-versus-host disease (GVHD), Inadequate Human resource, Inadequate equipped facility, cost of the service, Corruption, Lack of political will, Leadership incompétence,

Chisara Sylvestina Okolo,Ayodeji Olusola Dickson Olayanju 

strategic planing, Policy inconsistency, Lack of qualified personnel, Poor healthcare administration, Conflict of interest among health workers. The cost of Bone Marrow Transplantation is highly exorbitant to Nigerians and government need to play vital role towards funding part of the cost and alleviating the pain of sickle cell. Effective implementation of National Health Insurance Scheme and address corruption are the most efficient ways the cost would be subsidized. Appropriate information campaigns largely championed by maternal and child healthcare professionals would significantly contribute to raising the level of awareness and acceptance of Cord Blood donation. Training and re training of medical personnel cannot be over emphasized.

Download PDF - 86 Downloads

 
 

Some haematological parameters of welders exposed to oxyacetylene in Calabar, Nigeria.

Download PDF - 93 Downloads

 
 

Joyce Ezekiel Etura, Kenneth Igbor Ebi, Uwem Okon Akpan, Enosakhare Aiyudubie Asemota and Anthony Ogbonnaya Emeribe

AJLHTS: Original Paper

Abstract 

Introduction: Polymorphonuclear neutrophils (PMN) are the only leucocytes that are competent to produce large amounts of reactive oxygen species (ROS) to kill phagocytized bacteria. In leukaemia, we hypothesized this bactericidal function might be hampered. The goal of this study was to assess respiratory burst activity of PMN in leukaemia compared with non-leukaemic control subjects. We assessed the respiratory burst function of PMN as an effective defence against pathogens in leukaemia and control subjects

Materials and Methods: Peripheral blood samples were collected from leukaemia and control subjects (30 in each case) in lithium heparin anticoagulant containers. Our study cases were 30 leukaemia belonging to different subtypes (AML=12, CML=12, ALL=4 and CLL=2), 22 males and 8 females age range from 2.5-63 years (M±SEM, 28.0±3.4 years); the controls were 26 males and 4 females, age range from 17-53 years (M±SEM, 31.1±1.5 years), respectively. The respiratory burst activity was assessed using the nitroblue tetrazolium (NBT) dye reduction test on stimulated and un-stimulated PMN in leukaemia and control groups

Results: The cells purity in leukaemia was >99% using Turk’s solution and cells viability was >95% by Trypan blue dye exclusion test. The respiratory burst activity of PMNs showed a statistically significant increase (P<0.05) in controls compared with leukaemia subtypes. Similarly, comparison within leukaemia subtypes indicates

statistically significant increase (P<0.05) RBA in CML compared with AML, ALL and CLL, respectively

Conclusion: The respiratory burst activity of PMNs in leukaemia is variable with enhanced activity in CML subjects, while depressed in AML, ALL and CLL subtypes; suggesting impaired bactericidal capacities of PMNs in these diseases.

Download PDF - 93 Downloads

 
 

Respiratory Burst Activities of Peripheral Blood Neutrophils in Leukaemic Subjects in Northern Nigeria

Download PDF - 93 Downloads

 
 

Ajayi, O.l and Danladi S.B.

AJLHTS: Original Paper

Abstract 

Introduction: Polymorphonuclear neutrophils (PMN) are the only leucocytes that are competent to produce large amounts of reactive oxygen species (ROS) to kill phagocytized bacteria. In leukaemia, we hypothesized this bactericidal function might be hampered. The goal of this study was to assess respiratory burst activity of PMN in leukaemia compared with non-leukaemic control subjects. We assessed the respiratory burst function of PMN as an effective defence against pathogens in leukaemia and control subjects

Materials and Methods: Peripheral blood samples were collected from leukaemia and control subjects (30 in each case) in lithium heparin anticoagulant containers. Our study cases were 30 leukaemia belonging to different subtypes (AML=12, CML=12, ALL=4 and CLL=2), 22 males and 8 females age range from 2.5-63 years (M±SEM, 28.0±3.4 years); the controls were 26 males and 4 females, age range from 17-53 years (M±SEM, 31.1±1.5 years), respectively. The respiratory burst activity was assessed using the nitroblue tetrazolium (NBT) dye reduction test on stimulated and un-stimulated PMN in leukaemia and control groups

Results: The cells purity in leukaemia was >99% using Turk’s solution and cells viability was >95% by Trypan blue dye exclusion test. The respiratory burst activity of PMNs showed a statistically significant increase (P<0.05) in controls compared with leukaemia subtypes. Similarly, comparison within leukaemia subtypes indicates

Ajayi, O.I. and Danladi S.B. 

statistically significant increase (P<0.05) RBA in CML compared with AML, ALL and CLL, respectively

Conclusion: The respiratory burst activity of PMNs in leukaemia is variable with enhanced activity in CML subjects, while depressed in AML, ALL and CLL subtypes; suggesting impaired bactericidal capacities of PMNs in these diseases.

Download PDF - 93 Downloads

 
 

Cyclophosphamide, thalidomide and dexamethasone (CTD) as first-line therapy in multiple myeloma patients: an experience in a clinical haematology centre in Dakar, Senegal

Download PDF - 84 Downloads

 
 

El Hadji Daouda Niang, Fall Seynabou, Sarr Khadim, Camara Marieme Lolita, Dakono Aminata, Ndiaye Awa, Ciss Modou Moustapha, Thiam Amy, and Ndiaye Fatou Samba Diago

AJLHTS: Original Paper

Abstract 

Introduction 

Induction therapy followed by high-dose chemotherapy with autologous stem cell transplantation remains the gold standard for myeloma patients who can tolerate this treatment approach. In a developing country setting, in the absence of availability of bone marrow transplantation, the CTD protocol is an accessible treatment regimen whose efficacy and lower toxicity compared to the Melphalan Prednisone protocol has been reported. This protocol has been administered since 2018 in first line at the clinical haematology department of Dalal Jamm Hospital. It’s against this backdrop, that we perform this study to assess the efficacy of this CTD protocol in first line therapy

Methods 

We conducted a descriptive and analytical study including clinical, paraclinical and evolutionary data of 49 patients with MM treated during the period range from 01 September 2018 and 30 November 2021 with the CTD protocol of cyclophosphamide (500mg at D1, D8 and D15), dexamethasone (40mg weekly) and thalidomide (100mg/day) in 28-day cycles. Survival outcomes were estimated by the KaplanMeier method. 

Results 

The mean age was 62.3 ± 9.1 years and the sex ratio was 0.8. An advanced prognostic score at diagnosis was found in 73.5% of patients according to the Salmon and Durie score and in 32% according to the ISS. Overall remission was noted in 48.9%, of which 30.6% were in very good partial remission and partial remission in 12.2% of cases

Afr J Lab Haem Transf Sci 2022, 1(4): 183 – 189 

El Hadji Daouda Niang, Fall Seynabou, Sarr Khadim, Camara Marième Lolita 

Progression was noted in 2% of cases. The median survival was 29 months with an overall survival of 78% at 39 months. Treatment- related side effects were mainly peripheral neuropathy in 2% and anaemia in 6%. The median PFS was 27 months with a 72% survival at 39 months. An advanced age (≥ 65 years) is correlated with negative impact on survival (p=0.04). 

Conclusion 

Cyclophosphamide, thalidomide and dexamethasone give good outcome with less toxicity. Thus, it remains a first-line treatment alternative for newly diagnosed and lowincome patients.

Download PDF - 84 Downloads